Toluene-in-Hexane reference standard for resolving spectral bandwidth of UV/VIS spectrophotometers

Assessing the photometric and wavelength performance of UV/VIS spectrophotometers is a necessary part of keeping your laboratory in accordance with  GLP-, European Pharmacopeia (UP) or United States Pharmacopeia (USP) standards.

The spectral bandwith (SBW) refers to the physical slit-width of the spectrophotometer's monochromator, which has a direct impact on the instrument's resolution. In turn, spectral resolution is the ability to resolve two neighbouring peaks. Reconciling the resolution is an integral component in getting accurate wavelength and photometric readings on your spectrophotometer.

Recognizing the need for periodic assessment of SBW and pursuant to EP and USP requirements, FireflySci has expanded its range of calibration products to include this Toluene in Hexane standard.

Delivery includes:

• High-purity solution of 0.02% Toluene in Hexan
• High-purity Hexane blank

Suggested use:

1. The EP reommends comparing the peak maximum at 269nm and the peak minimum at 266nm.
2. Determine the resolution by running the Toluene in Hexane against the Hexane blank between 260 and 270nm and calculate the peak minima and maxima.
3. Finally, calculate the peak-to-trough ratios and compare the results with the instruments reference values. If they are considerable lower, you may wish to contact your instrument manufacturer.  

Please note that this standard must be recalibrated annually according to NIST guidelines.